Nanoscope Software 6.13 Download 16
MultiMode 8 – nanomechanics modes. In order to analyze interesting mechanical properties, such as adhesion, Young´s modulus or even the structure of thin films, MultiMode 8 ™ enables innovative. ScanAsyst ™ technology is a revolutionary imaging optimization mode, which enables both expert and novice users to access the most advanced applications and modes to obtain research-quality results easier and faster. The MultiMode 8 ™ features the new PeakForce QNM ™, Veecos proprietary, quantitative nanomechanical property mapping, which delivers more accurate, repeatable results for modulus and adhesion measurements while also helping to preserve sample and probe integrity. Finally, new NanoScope Version 8.1 software offers a simplified interface and faster, more powerful tools for data gathering and off-line analysis.
No matter if you download the stand-alone software or use the Nanoscope software embedded on the scanner, you need some additional tools and a proper data collection method. One of the methods to record surface topography is to use the vertical movement of the cantilever to construct images. The calculation of topography is done by taking an offset point, determining the distance (in the case of a shape, length or depth) and adding it to the previous point. The result of this calculation is stored as a matrix: one row represents one point of the data and one column represents the distance of the cantilever in the Z-direction. The distance is typically around 5×10µm. Of course, the minimum distance needed to get a usable image depends on the size of the tip, the size of the sample and the distance the AFM is moved on the sample. One can retrieve the original position of the cantilever in the matrix at any time. This is needed to know the original position of the surface. It is also the reason we calculate Z-positions and not distances. A sample is either height or Z-position data. To determine the height or the Z-position of a certain point, one has to calculate the distance of the point in the Z-direction to the original position. In a second step this is done for the upper line of the matrix (representing the Z-position). Depending on the amount of points recorded in the matrix, this can take a while. There are two problems which happen in most cases when recording this type of data. 1. One has to scan the area multiple times to get enough points. This results in problems, because the cantilever is always at the same height or Z-position on the surface and it is not possible to move the tip to the surface while recording. This means the distance needed to get a usable image is always the same, which is not the case in reality. This is the reason we calculate height and not distance on this thread. 2. In some cases, the algorithm to determine the original position seems to fail. It then becomes a trial and error method. I rewrote the whole algorithm and improved it over the last years. I hope it is reliable enough, but I have not yet tested it with a long scan of a surface. There is a new version of our XE-Nano software coming out. When it is finished, you will be able to download it for free on our web site. It also contains functions to visualize height and Z-positions data and assemble them nicely.
Overall, I also recommend checking out the np-protocols page.
A previously submitted and filtered scRNA-seq dataset comprising the ETX and natural embryos was downloaded from the Gene Expression Omnibus repository ( GSE161947 ) 29 . The count matrix was loaded into Seurat version 3 (ref. 55 ), the fraction of counts mapping to mitochondrial genes was computed and the object was then log-normalized to a scale factor of 10,000. The 2,000 most variable genes were computed, the object was scaled and the percentage of mitochondrial counts was regressed out. PeakForce QNM: The new PeakForce QNM option provides researchers with the ability to perform quantitatively nanomechanical property mapping using the new PeakForce QNM technique. Investigators can adjust the force-volume bias, the fit bias, the offset bias, the calibration parameters, the contact stiffness, and the maximum penetration depth to fit these quantities to the measured data. Resolved indentations are automatically extracted for all cantilever positions. The PeakForce QNM option uses the new PeakForce QNM software described in the following paper: “Temporal and spatial comparison of indentation stiffness of native and partially mineralized kidney stones using adaptive PeakForce QNM” . Nanoscope Version 8.1 software: Nanoscope 8.1 software offers a refreshed user interface and brings new functionality to the MultiMode platform. Faster measurement speeds enable users to analyze more samples in less time. In addition, new measurements such as force amplitude and deflection, and annotations such as line profiles and profile fits are now available. Maximum deformation and contact point cannot be set directly by the user in the new Nanoscope 8.1 software. This functionality is now only accessible in Scanasyst or automatic mode. To adjust these measurements, users can make use of the relevant controls within the ScanAsyst or automatic modes. 5ec8ef588b