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Background ========== Monoclonal antibodies have been used to target cytokines and proinflammatory factors to treat a range of diseases. The discovery of these proteins and the therapeutic potential of monoclonal antibodies has created a significant role for the development of recombinant antibody fragments. We were interested in the development of monoclonal antibodies against proinflammatory cytokines and their ability to reduce levels of these cytokines in vivo. Methods ======= We have investigated the potential of the recombinant monoclonal antibodies in reducing the inflammation in an immunological mouse model of arthritis. The antibodies were injected into the mice intraperitoneally, and a control group was administered 10 mg of the anti-IL-10 antibody clone H.10, which we have previously reported to reduce immune-mediated diseases. During the course of treatment and within 48 hours after the last injection, we measured the levels of IL-17, TNF-α and IL-1β cytokines in the serum by ELISA. Results ======= Our results show that the recombinant monoclonal antibodies reduced the levels of the cytokines IL-17, TNF-α and IL-1β in the mice sera with two different patterns of administration, with the effect of the antibodies being more pronounced when the antibodies were administered as early as possible during the treatment time-period and with a single administration. Conclusions =========== These data confirm the potential of recombinant monoclonal antibodies as therapeutic agents in the prevention of autoimmune diseases, based on our results they have the potential of being used as a treatment for arthritis and autoimmune disease. Effect of different surface treatments on the roughness, hardness and fluoride uptake of dental enamel. The objective of the present in vitro study was to determine the effect of different surface treatments on the roughness, hardness and fluoride uptake of human enamel. Thirty-five human premolars were sectioned mesiodistally to obtain a flat surface on the enamel. The specimens were randomly assigned to five groups (n = 7), each group representing different surface treatments. Surface treatments included air abrasion, sandblasting, phosphoric acid etching, 10% polyacrylic acid gel application and ultra sound, followed by silane priming. Each specimen was subjected to roughness, microhardness and fluoride uptake measurements (quantitative micro-photomacrography). Surface roughness increased significantly in the untreated 3da54e8ca3